文献类型：期刊文献

英文题名：Highly sensitive and fast responsive fluorescence turn-on chemodosimeter for Cu2+ and its application in live cell imaging

作者：Yu, Mengxiao[1]; Shi, Mei[1]; Chen, Zhigang[1]; Li, Fuyou[1]; Li, Xinxin[2]; Gao, Yanhong[3]; Xu, Jia[1]; Yang, Hong[1]; Zhou, Zhiguo[1]; Yi, Tao[1]; Huang, Chunhui[1]

机构：[1] Department of Chemistry, Laboratory of Advanced Materials, Fudan University, 220 Handan Road, Shanghai 200433, China; [2] School of Materials Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; [3] Hospital of Xinhua, Medicine School, Shanghai Jiaotong University, 1665 Kongjiang Road, Shanghai 200092, China

年份：2008

卷号：14

期号：23

起止页码：6892

外文期刊名：Chemistry - A European Journal

收录：EI(收录号：20084311649559)

语种：英文

外文关键词：Trace elements - Copper - Fluorescence quenching - Redox reactions - Dosimetry - Fluorescence imaging - Probes - Metal ions - Chlorine compounds - Lanthanum compounds

摘要：A rhodamine B derivative 4 containing a highly electron-rich S atom has been synthesized as a fluorescence turn-on chemodosimeter for Cu2+. Following Cu2+-promoted ring-opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were observed upon addition of Cu2+; this suggests that chemodosimeter 4 effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu2+. Importantly, 4 can selectively recognize Cu2+ in aqueous media in the presence of other trace metal ions in organisms (such as Fe3+, Fe2+, Cu+, Zn2+, Cr 1+, Mn2+, Co2+, and Ni2+), abundant cellular cations (such as Na+, K+, Mg2+, and Ca2+), and the prevalent toxic metal ions in the environment (such as Pb2+ and Cd2+) with high sensitivity (detection limit ≤10ppb) and a rapid response time (≤1 min). Moreover, by virtue of the chemodosimeter as fluorescent probe for Cu2+, confocal and two-photon microscopy experiments revealed a significant increase of intracellular Cu 2+ concentration and the subcellular distribution of Cu2+, which was internalized into the living HeLa cells upon incubation in growth medium supplemented with 50 μM CuCl2 for 20 h. ? 2008 Wiley-VCH Verlag GmbH & Cu. KGaA.