文献类型：期刊文献

中文题名：应用洗脱-推挤逆流色谱法高效分离制备人参皂苷Rg_1、Rf及Rd

英文题名：Elution-extrusion counter-current chromatography for preparation of ginsenoside-Rg_1,Rf and Rd

作者：程屹俊[1,2];梁琼麟[2];王义明[2];伍军[3];罗国安[1,2]

机构：[1]华东理工大学中药现代化工程中心,上海200237;[2]清华大学化学系,北京100084;[3]广州香雪制药股份有限公司,广东广州510530

年份：2012

卷号：34

期号：1

起止页码：89

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：高速逆流色谱;洗脱-推挤逆流色谱;人参皂苷Rg1;人参皂苷Rf;人参皂苷Rd

外文关键词：high speed counter-current chromatography; elution-extrusion counter-current chromatography; ginsenoside-Rg1; ginsenoside-Rf; ginsenoside-Rd

摘要：目的运用洗脱-推挤逆流色谱技术(EECCC)分离制备人参皂苷Rg1、Rf及Rd。方法以乙酸乙酯-正丁醇-0.1%甲酸水(2︰1︰3,v/v)为溶剂体系,上相为固定相,下相为流动相,仪器转速为1 250 r/min,操作体积流量为40 mL/min,切换体积为2 400 mL。结果在此条件下,经一步纯化从300 mg样品中分离纯化得到71 mg人参皂苷Rg1、21 mg人参皂苷Rf及53 mg人参皂苷Rd,经高效液相色谱分析,纯度分别达到96.2%、94.3%和95.1%。结论 EECCC制备方法快速,简单。对于分离分配系数高的物质十分有效。
AIM To use elution-extrusion counter-current chromatography（EECCC） to separate and prepare ginsenoside-Rg1,Rf and Rd in Ginseng Radix et Rhizoma.METHODS Ethyl acetate-n-butanol-0.1%formic acid（2∶ 1∶ 3,v/v） was chosen as the solvent system.The upper phase was used as stationary phase,and the lower phase used as mobile phase.The apparatus was rotated at 1 250 r/min,and the mobile（lower） phase was pumped into the column at 40 mL/min.The switch volume（VCM） was 2 400 mL.RESULTS Under the chosen condition,the preparetion yielded 71 mg,21 mg and 53 mg of ginsenoside-Rg1,Rf and Rd with a purity of 96.2%,94.3% and 95.1% by HPLC from 300 mg crude sample,respectively.CONCLUSION EECCC is a rapid and simple counter-current chromatography technique,which is effective for separation and purification of the solutes with larger partition coefficient（KD） values.