文献类型：期刊文献

英文题名：Supramolecularly Engineered Gold Nanozymes on Spherical Polyelectrolyte Brushes for Sensitive Colorimetric Detection of H2o2 and Ascorbic Acid

作者：Liu, Liqun[1]; Liu, Henghui[1]; Zhou, Na[1]; Xu, Yisheng[1]; Guo, Xuhong[1,2]; Wang, Jie[1]

机构：[1] State-Key Laboratory of Chemical Engineering, School of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250248303)

语种：英文

外文关键词：Catalyst activity - Chemical detection - Color - Colorimetry - Enzymes - Food products - Food safety - Gold compounds - Gold nanoparticles - Spheres

摘要：Gold-based nanozymes have emerged as promising alternatives to natural enzymes owing to their exceptional stability, biocompatibility, and tunable catalytic activity. Nevertheless, their relatively low intrinsic enzyme-mimicking efficiency remains a challenge. In this work, a supramolecular nanozyme (AuNP@CDs) was constructed via the assembly of gold nanoparticles with cationic β-cyclodextrin (HMA-β-CD), and subsequently immobilized onto negatively charged spherical polyelectrolyte brushes (SPBs) through electrostatic interaction, forming a hybrid nanozyme denoted as SPB@AuNP@CDs. The immobilized nanozyme exhibited significantly enhanced peroxidase-like activity, along with markedly improved affinity for H2O2 compared to both free AuNP@CDs and horseradish peroxidase (HRP). Mechanistic investigations revealed a catalytic pathway shift from hydroxyl radical generation to electron transfer upon SPBs-assisted immobilization. Based on this hybrid nanozyme, a robust colorimetric sensing platform was developed for the rapid, sensitive, and selective detection of hydrogen peroxide and ascorbic acid. The sensor demonstrated excellent anti-interference capability in the presence of various coexisting substances. This work not only provides a new strategy for enhancing nanozyme activity through supramolecular and interfacial engineering but also offers a promising platform for biosensing, food safety, and environmental monitoring applications. ? 2025, The Authors. All rights reserved.