文献类型：期刊文献

中文题名：高温二维液相色谱系统的构建及其评价

英文题名：Construction and Evaluation of High Temperature Two-Dimensional Liquid Chromatography System

作者：李笃信[1];魏远隆[1];宋伦[1];李彤[2];杜一平[1];张维冰[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]大连依利特分析仪器有限公司,大连116023

年份：2009

卷号：67

期号：21

起止页码：2481

中文期刊名：化学学报

外文期刊名：Acta Chimica Sinica

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000272761100014)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(No.20675083);"973"国家重大基础研究发展规划(No.2007CB914100);"十一五"国家科技支撑计划(No.2006BAF07B03)资助项目

语种：中文

中文关键词：高温;二维液相色谱系统;离子交换色谱/反相色谱;多肽

外文关键词：high temperature; two-dimensional liquid chromatography; ion exchange/reversed phase liquid chromatography; peptide

摘要：在二维液相色谱中,第二维的分离速度是制约其发展的重要因素.升高色谱柱温度可以有效降低流动相粘度,加快溶质在两相间的传质速率,有效加快分析速度.以离子交换色谱法(WAX)为第一维分离模式和反相色谱法(RP)为第二维分离模式,十通阀和两个捕集柱为接口,通过将第二维色谱柱温度升高到80℃和提高流量到3 mL/min,构建了高温WAX/RP二维液相色谱系统.以4种标准蛋白的酶解物为样品评价系统的分离性能,第一维共有33个馏分被捕集并导入到第二维分析,高温二维液相色谱系统识别出187个色谱峰.
The separation speed is very importantto the development of two dimensional liquid chromatography. The viscosity of the mobile phase decreases and the analyte diffusivity increases at higher temperature. An ion exchange chromatography column was used as the first dimension mode and reversed phase chromatography as the second dimension mode, which were connected by the interface consisting of a ten-port valve and two trap columns. A high temperature two dimensional weak anion exchange （WAX）/ reversed phase （RP） liquid chromatography system was developed by the second dimensional column operated at 80 ℃ and higher flow rate up to 3 mL/min. A tryptic digest of 4 proteins was separated on this sys- tem. 33 fractions from the first dimension were introduced to the second dimension by the interface. 187 peaks were separated by the high temperature two dimensional liquid chromatography system.