文献类型：期刊文献

中文题名：金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析

英文题名：Metal/Matrix Enhanced Time-of-Flight Secondary Ion Mass Spectrometry for Single Cell Lipids Analysis

作者：李好问[1];华鑫[1];龙亿涛[1]

机构：[1]结构可控先进功能材料及其制备教育部重点实验室,华东理工大学化学与分子工程学院,上海200237

年份：2018

卷号：46

期号：1

起止页码：61

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;EI(收录号：20181404985059);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000427609600009)】；北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：上海市青年科技英才扬帆计划(No.17YF1403000);上海市自然科学基金(No.17ZR1407700);中国博士后科学基金(No.2017M611470)资助

语种：中文

中文关键词：单细胞分析;脂质;飞行时间-二次离子质谱;金属/基质增强;质谱成像

外文关键词：Single cell analysis;Lipids;Time-of-flight secondary ion mass spectrometry;Noble metal/matrix enhancement;Mass spectrometry imaging

摘要：单细胞中化学成分的分析对细胞生长、信号转导、凋亡等生理过程意义重大。飞行时间-二次离子质谱(SIMS)是一种高灵敏的表面质谱成像技术,具有较高的空间分辨率,已被用于细胞及微区域分析。然而,生物有机分子较低的离子化效率限制了To F-SIMS在单细胞分析中的广泛应用。本研究采用金属/基质增强方法,明显提高了脂质的离子化产率。镀金硅片上磷脂酰胆碱PC(40:0)标样在加入基质后,准分子离子峰强度增大为硅片上的65倍。相应基底上单细胞表面脂质信号同时增强,但由于细胞的不规则形貌以及复杂化学环境影响,脂质信号增强幅度较小。在此基础上,使用延时提取(Delayed extraction,DE)模式克服了细胞形貌带来的影响,进一步增强脂质信号,并获得了高质量的单细胞脂质成像图。此方法为研究细胞代谢及细胞-环境相互作用提供了新的途径。
The chemical components analysis of single cell is important for understanding of physiological processes such as cell growth,signal transduction and apoptosis.Time-of-flight secondary ion mass spectrometry(ToF-SIMS)is a sensitive surface analysis technique with high spatial resolution and can be used for single cell and micro-area analysis.However,relatively low ionization yield of biomolecules limited its wide application in single cell analysis.Herein,we used metal substrate and matrix material to enhance the ionization yield of lipids.The signal intensity of the phosphatidylcholine PC(40:0)casted on the matrix/gold coated silicon substrate was65times higher than that on the silicon wafer.Signal enhancement of phosphatidylcholine PC(34:1)on the single cell surface cultured on matrix/gold coated silicon substrate was observed as well.Due to the influence of irregular topography and complex chemical environment of cell,the increase of lipids signal was smaller.Delayed extraction mode of ToF-SIMS overcame the effects of cell topography,leading to further enhancement of the signal intensity of lipids.Meanwhile,simultaneous high spatial resolution of chemical imaging and high mass resolution of the mass spectra of single cells were obtained.Our strategies provided new insights into the study of cell metabolism and cell-environment interactions.