文献类型：期刊文献

英文题名：A GeneralStrategy for Developing Si-Rhodamine-BasedFluorogenic Dyes for Advanced Bioimaging and Biosensing

作者：Chen, Zhengda[1];Shi, Yajie[2];Jiang, Li[1,2];Yin, Yuxin[2];Yang, Lipeng[1];Huang, Xinyi[1];Jiang, Ling[2];Guo, Wenjie[1];Liu, Xiaoyu[2];Fu, Rong[2];Liu, Huining[2];Bao, Bingkun[1];Lin, Qiuning[1];Zhao, Yuzheng[2];Chen, Xianjun[2];Yang, Yi[2,3];Zhu, Linyong[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China;[2]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China;[3]Frontiers Med Ctr, Tianfu Jincheng Lab, Chengdu 610200, Peoples R China

年份：2026

卷号：148

期号：27

起止页码：28764

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20262921138241);Scopus(收录号：2-s2.0-105045133352);WOS:【SCI-EXPANDED(收录号:WOS:001810431700001)】；

基金：This research was supported by the National Key Research and Development Program of China (2024YFA0917700, 2022YFC3400100, 2024YFA1803501, and 2023YFA1802000), NSFC (32121005, 92357308, 22437001, 92581203, 32250009, and 22507071), STI2030-Major Projects (2021ZD0202200 and 2021ZD0202203), the grant from the Tianfu Jincheng Laboratory (TFJCPI20250016), the Shanghai Municipal Education Commission (2021 Sci & Tech 03-28), the Natural Science Foundation of Shanghai (23J21900400 and 24ZR1432500), the Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China (JYB2025XDXM404), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Bioimaging - Dyes - Fluorescence imaging - Silicon compounds - Stimulated emission

摘要：Si-rhodamine (SiR) and its derivatives have advanced bioimaging research because of their high photostability and bright near-infrared (NIR) emissions. However, the rational design of SiR derivatives with high fluorogenicity remains challenging. Herein, we report a general strategy for developing high-performance fluorogenic SiR derivatives, termed SFs, by incorporating a molecular rotor at the meso position of the SiR scaffold. This design enables a twisted intramolecular charge transfer (TICT)-based mechanism for fluorescence on/off switching, which results in the achievement of a maximum fluorescence on/off ratio of more than 3000-fold. Chloroalkane (CA)-conjugated SFs exhibit high cellular brightness and outstanding photostability, enabling robust and high-contrast fluorescent labeling of HaloTag in live cells and in vivo. Their suitability for use in stimulated emission depletion microscopy enables advanced fluorescence imaging with subdiffraction resolution in live cells. We demonstrate the ability of SFs to construct chemigenetic Ca2+ indicators with large dynamic ranges, enabling sensitive detection of intracellular and in vivo Ca2+ dynamics using both fluorescence intensity and lifetime. This work establishes a general design strategy for NIR fluorogenic dyes, thus opening new avenues for advanced bioimaging and biosensing.