文献类型：期刊文献

英文题名：Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

作者：Chang, Kang[1];Guo, Tongtong[2];Li, Pengfei[1];Liu, Yin[2];Xu, Yufang[1];Fang, Yuda[2];Qian, Xuhong[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plan Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000382396000001)】；

基金：This work is financially supported by the National Natural Science Foundation of China (Grants 21236002), the National High Technology Research and Development Program of China (863 Program 2011AA10A207) and the National Key Laboratory of Plant Molecular Genetics.

语种：英文

摘要：Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identification and real time tracking of NOS-like enzymes in plant by fluorescence sensing. It could bind NOS-like enzyme efficiently and enter the cell successfully. In vivo fluorescence response results directly proved that NOS-like enzymes did exist in tobacco leaf and would be stimulated by pathogen infection, which also provided a useful chemical tool for the study of the function of NOS-like enzyme in plants.