文献类型：期刊文献

英文题名：Glucose monitoring in living cells with single fluorescent protein-based sensors

作者：Hu, Hanyang[1,3];Wei, Yufeng[1,3];Wang, Daocheng[1,3];Su, Ni[1,3];Chen, Xianjun[1,3];Zhao, Yuzheng[1,3];Liu, Guixia[3];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai 200031, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：8

期号：5

起止页码：2485

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20180404679171);WOS:【SCI-EXPANDED(收录号:WOS:000422864800029)】；

基金：This work was supported by National Key Research and Development Program of China (2017YFA050400 to Y. Y., 2017YFC0906900 to Y. Zhao), NSFC (31225008, and 31470833 to Y. Y., 31722033, 91649123, and 31671484 to Y. Zhao), the Shanghai Science and Technology Commission (14XD1401400 and 16430723100 to Y. Y., and 15YF1402600 to Y. Zhao), Young Elite Scientists Sponsorship Program by Cast (to Y. Zhao), Shanghai Young Top-notch Talent (to Y. Zhao), the State Key Laboratory of Bioreactor Engineering (to Y. Y.), and the Fundamental Research Funds for the Central Universities (to Y. Y. and Y. Zhao).

语种：英文

外文关键词：Proteins - Escherichia coli - Cells - Cytology - Fluorescence - Metabolism - Glucose sensors

摘要：Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. However, the sensitive fluctuation of glucose in living cells is difficult to monitor. Thus, we developed a series of ratiometric, highly responsive, single fluorescent protein-based glucose sensors of wide dynamic range by combining a circularly permuted yellow fluorescent protein with a bacterial periplasmic glucose/galactose-binding protein. We used these sensors to monitor glucose transport in living Escherichia coli cells, and found that the cells take up glucose within 10 min to maintain physiological glucose levels, and observed the differences in glucose uptake and glucose metabolism between wild-type and Mlc knockout cells. These sensors can be specific and simple tools for glucose detection in vitro and non-invasive tools for real-time monitoring of glucose metabolism in vivo.