文献类型：期刊文献

英文题名：Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite

作者：Wu, Ting[1];Shi, Jiani[1];Zhang, Chuanjing[1];Zhang, Lingfan[1];Du, Yiping[1]

机构：[1]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2016

卷号：1008

起止页码：234

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20155001666602);WOS:【SCI-EXPANDED(收录号:WOS:000368205500031)】；

基金：This work was supported by the National Science Foundation of China (21205041, 21407050), the Fundamental Research Funds for the Central Universities (222201314039), General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (20141K077), and a grant of Laboratory team construction program for universities in Shanghai.

语种：英文

外文关键词：Phosphopeptide enrichment; IMAC; Chitosan; MALDI TOF MS

摘要：Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS). Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain. The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method. The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum. Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS. Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides. (C) 2015 Elsevier B.V. All rights reserved.