文献类型：期刊文献

英文题名：Extraction of Acetoin from Fermentation Broth Using an Acetone/Phosphate Aqueous Two-Phase System

作者：Sun, Jianan[1];Rao, Ben[1];Zhang, Liaoyuan[2];Shen, Yaling[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]FuJian Agr & Forestry Univ, Sch Life Sci, Fuzhou, Peoples R China

年份：2012

卷号：199

期号：11

起止页码：1492

外文期刊名：CHEMICAL ENGINEERING COMMUNICATIONS

收录：;EI(收录号：20123315337687);WOS:【SCI-EXPANDED(收录号:WOS:000307927000008)】；

基金：This work was supported by the Chinese National Program for High Technology and Development (863 Program, No. 2006AA02Z243), Shanghai Leading Academic Discipline Project (No. B505), and State Key Laboratory special fund (No. 2060204).

语种：英文

外文关键词：Acetoin; Acetone/phosphate; Aqueous-two phase system; Fermentation; Partition coefficient; Recovery

摘要：An aqueous two-phase system (ATPS) consisting of acetone and phosphate was used to extract acetoin from fermentation broth. The influence of phase composition on partition of acetoin was investigated. When the filtered fermentation broth was used, relatively high partition coefficient (22.3) and recovery coefficient (96.4%) of acetoin were obtained by a system composed of 30% (w/w) acetone and 35% (w/w) dipotassium hydrogen phosphate. Then the system was applied to extract unfiltered fermentation broth directly, and the recovery coefficient of acetoin was 94.3%. Simultaneously, the byproduct 2,3-butanediol could also be extracted with the recovery coefficient of 93.5%. In addition, the removal of residual sucrose, cells, proteins, and prodigiosin from the fermentation broth was studied, and the removal ratios of these impurities were all above 85%. Ultimately, the recovery of phosphate in the bottom phase was explored, and the recovery coefficient could reach 93.7% through pH adjustment and dilution crystallization. The recovered phosphate also showed good ATPS extraction ability. This method provides a new possible way for the separation of acetoin from fermentation broth.