文献类型：期刊文献

英文题名：Responses of Microbial Community Composition to Temperature Gradient and Carbon Steel Corrosion in Production Water of Petroleum Reservoir

作者：Li, Xiao-Xiao[1,2];Yang, Tao[1,2];Mbadinga, Serge M.[1,2,3];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China;[4]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000417045500001)】；

基金：This work was supported by the National Science Foundation of China (No. 41530318, 41673084), the Research Foundation of Shanghai (No. 15JC1401400), and the Fundamental Research Funds for the Central Universities of China (No. 222201 717017).

语种：英文

外文关键词：microbial induced corrosion; temperature gradient; biocorrosion; mesophilic; thermophilic and hyperthermophilic microorganisms; oil reservoir

摘要：Oil reservoir production systems are usually associated with a temperature gradient and oil production facilities frequently suffer from pipeline corrosion failures. Both bacteria and archaea potentially contribute to biocorrosion of the oil production equipment. Here the response of microbial populations from the petroleum reservoir to temperature gradient and corrosion of carbon steel coupons were investigated under laboratory condition. Carbon steel coupons were exposed to production water from a depth of 1809 m of Jiangsu petroleum reservoir (China) and incubated for periods of 160 and 300 days. The incubation temperatures were set at 37, 55, and 65 degrees C to monitoring mesophilic, thermophilic and hyperthermophilic microorganisms associated with anaerobic carbon steel corrosion. The results showed that corrosion rate at 55 degrees C (0.162 +/- 0.013 mm year 1) and 37 degrees C (0.138 +/- 0.008 mm year 1) were higher than that at 65 degrees C (0.105 +/- 0.007 mm year 1), and a dense biofilm was observed on the surface of coupons under all biotic incubations. The microbial community analysis suggests a high frequency of bacterial taxa associated with families Porphyromonadaceae, Enterobacteriaceae, and Spirochaetaceae at all three temperatures. While the majority of known sulfate-reducing bacteria, in particular Desulfotignum, Desulfobulbus and Desulfovibrio spp., were predominantly observed at 37 degrees C; Desulfotomaculum spp., Thermotoga spp. and Thermanaeromonas spp. as well as archaeal members closely related to Thermococcus and Archaeoglobus spp. were substantially enriched at 65 degrees C. Hydrogenotrophic methanogens of the family Methanobacteriaceae were dominant at both 37 and 55 degrees C; acetoclastic Methanosaeta spp. and methyltrophic Methanolobus spp. were enriched at 37 degrees C. These observations show that temperature changes significantly alter the microbial community structure in production fluids and also affected the biocorrosion of carbon steel under anaerobic conditions.