文献类型：期刊文献

英文题名：Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in Vivo Imaging of an Ovarian Cancer Biomarker

作者：Yao, Defan[1,2];Lin, Zhi[3];Wu, Junchen[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

年份：2016

卷号：8

期号：9

起止页码：5847

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20161202113994);WOS:【SCI-EXPANDED(收录号:WOS:000371945700014)】；

基金：We thank the National Basic Research 973 Program (2013CB733700), NSFC/China (91529101 and 21572057), the Program of Introducing Talents of Discipline to Universities (B16017), and the Fundamental Research Funds for the Central Universities (WJ1213007) for financial support. We also thank Prof. He Tian for his constructive suggestions.

语种：英文

外文关键词：lysophosphatidic acid; biomarker; polarity-sensitive; near-infrared; probe

摘要：Lysophosphatidic acid (LPA, cutoff values >= 1.5 mu M) is an effective biomarker for early stage ovarian cancer. The development of selective probes for LPA detection is therefore critical for early clinical diagnosis. Although current methods have been developed for the detection of LPA in solution, they cannot be used for tracking LPA in vivo. Here, we report a near-infrared (NIR) fluorescent probe that can selectively respond to LPA based on polarity-sensitive emission at a very low detection limit of 0.5 mu M in situ. This probe exhibits a marked increase of fluorescence at 720 nm upon binding to LPA, allowing the direct visualization of LPA in vitro and in vivo without interference from other biomolecules. Moreover, the probe containing two arginine-glycine-aspartic acid units can be efficiently taken up by cancer cells based on an alpha(v)beta(3) integrin receptor targeting mechanism. It also exhibits excellent biocompatibility and high pH stability in live cells and in vivo. Confocal laser scanning microscopy and flow cytometric imaging of SKOV-3 cells have confirmed that our probe can be used to image LPA in live cells. In particular, its NIR turn-on fluorescence can be used to effectively monitor LPA imaging in a SKOV-3 tumor-bearing mouse model. Our probe may pave the way for the detection of cancer-related biomarkers and even for early stage cancer diagnosis.