文献类型：期刊文献

中文题名：响应面法优化N-乙酰氨基葡萄糖发酵液精制纯化工艺

英文题名：Optimization of Impurity Removal Process of N-acetylglucosamine Fermentation Broth by Response Surface Method

作者：刘硕[1,2];刘鲁杰[1,2];王永红[1];赵黎明[1,2]

机构：[1]华东理工大学生物工程学院,上海200237;[2]华东理工大学发酵工业分离提取技术研发中心,上海200237

年份：2023

卷号：45

期号：7

起止页码：1

中文期刊名：化学试剂

外文期刊名：Chemical Reagents

收录：CSTPCD;;北大核心:【北大核心2020】；

语种：中文

中文关键词：响应面分析法;发酵液;NAG;离子交换树脂;纯化

外文关键词：response surface analysis;fermentation broth;NAG;ion exchange resin;purification

摘要：为从发酵液中精制N-乙酰氨基葡萄糖(NAG),以预处理的NAG发酵液为研究对象,开发NAG发酵液精制纯化工艺,筛选适用于NAG发酵液精制纯化的树脂,考察树脂用量、上样浓度、温度、料液流速对发酵液脱盐率、脱色率与NAG回收率的影响,在单因素实验的基础上,采用响应面分析法对NAG发酵液的精制纯化工艺进行优化。从6种树脂中,筛选出的DS-1树脂对发酵液脱盐效果最优、HS-3树脂对发酵液脱色效果最优,优化后的最佳条件为阴阳离子交换树脂体积比为0.35∶1、上样浓度为65 mg/mL、温度为41℃,此时发酵液脱盐率为96.9%,脱色率为79.5%,NAG回收率为84.5%,NAG结晶纯度可达99.8%。该精制纯化工艺可以有效地去除降低NAG品质的无机盐和色素,提高NAG纯度与结晶率并减少母液量。
In order to refine N-acetylglucosamine(NAG)from fermentation broth,the pretreated NAG fermentation broth was utilized to develop the purification process of NAG fermentation broth.The suitable resins for purification of NAG fermentation liquor were screened,and the effects of resin dosage,sample concentration,temperature and solid-liquid flow rate on the desalting rate,decolorization rate and NAG recovery rate of the fermentation liquor were investigated.On the basis of single factor experiments,response surface analysis was used to optimize the purification process of NAG fermentation liquid.Among the six resins,DS-1 resin was selected to have the best desalting effect on the fermentation solution,and HS-3 resin had the best decolorizing effect on the fermentation solution.The best conditions were the volume ratio of anion/cation exchange resin 0.35∶1,sample concentration 65 mg/mL,temperature 41℃.Thus,the desalting rate and decolorization rate of fermentation broth were 96.9%and 79.5%,respectively,the recovery rate of NAG was 84.5%,and the crystal purity of NAG could reach 99.8%.The purification process can effectively remove the inorganic salts and pigments which reduce the quality of NAG,improve the purity and crystallization rate of NAG and reduce the amount of mother liquor.