文献类型：期刊文献

英文题名：Study of matrix effects for liquid chromatography-electrospray ionization tandem mass spectrometric analysis of 4 aminoglycosides residues in milk

作者：Wang, Yuan[1];Li, Shaohui[1];Zhang, Feifang[1];Lu, Yifeng[1];Yang, Bingcheng[1];Zhang, Feng[2];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, 267 BO,130 Meilong RD, Shanghai 200237, Peoples R China;[2]Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing 100123, Peoples R China

年份：2016

卷号：1437

起止页码：8

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20160701945204);WOS:【SCI-EXPANDED(收录号:WOS:000371556700002)】；

基金：The research was sponsored by National Natural Science Foundation of China (Nos. 21322502, 21477037). We also thank for the support from Outstanding young talent cultivation fund from ECUST and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Matrix effect; Aminoglycosides; Sample preparation; Consecutive SPE; Milk; LC-MS/MS

摘要：Matrix effect (ME) is always a major issue for the development of LC-MS/MS method. ME resulting from co-eluting residual matrix components can affect the ionization efficiency of target analytes, leading to quantification errors of the analytes of interest. The present work evaluates MEs of milk samples on simultaneous analysis of four aminoglycosides residues via LC-ESI/MS/MS including streptomycin, dihydrostreptomycin, spectinomycin and kanamycin. Approaches to reduce MEs were examined: optimization of the sample preparation, sample dilution and lower flow rate used. Three commercial sorbents were tested including Oasis MCX, Oasis HLB and Oasis WCX. WCX behaved better for all analytes, but high MEs (80.8-134.9%) were obtained. Therefore, a consecutive SPE of tC18-WCX was found to effectively reduce ME. Milk samples from different manufacturers were analyzed and low MEs (85.6-112.9%) were obtained. (C) 2016 Elsevier B.V. All rights reserved.