文献类型：期刊文献

中文题名：拉曼光谱成像技术在药物经皮渗透行为考察中的应用

英文题名：Applications of Raman Spectroscopy and Imaging Technology in Investigations on Percutaneous Penetration Behaviors of Drugs

作者：沈心怡[1];胡韵池[1];熊訚然[1];康燕[1];杜一平[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2023

卷号：54

期号：5

起止页码：683

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;EI(收录号：20241315795273);北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

语种：中文

中文关键词：拉曼光谱;多元线性回归;经皮渗透;可视化分析

外文关键词：Raman spectroscopy;multiple linear regression;percutaneous penetration;visual analysis

摘要：该研究将拉曼光谱成像技术应用于药物分子透皮渗透分析,通过面扫描技术采集离体皮肤剖面的拉曼光谱,采用多元线性回归建立目标物浓度与信号强度的半定量分析模型。再将浓度贡献系数以热图的形式呈现,得到了目标物在皮肤中的浓度分布信息,实现了目标物浓度分布的可视化分析。以水杨酸为目标物,采用拉曼光谱成像技术研究其在猪皮肤中的渗透行为,2次平行试验中角质层中的目标物浓度贡献系数为0.231%和0.275%,活性表皮层中为3.1×10^(–4)和2.4×10^(–4)。将拉曼光谱成像结果和HPLC结果进行对比分析,可见2种方法得到的目标物浓度变化趋势一致,表明采用拉曼光谱成像技术得到的浓度贡献系数可用于半定量分析,为研究目标物经皮吸收特性、处方优化等提供了新的技术手段。此外,还采用该法考察了不同相对分子质量透明质酸在猪皮中的渗透分布情况,同样实现了皮肤中大分子分布的可视化分析。
Raman spectroscopy and imaging technology were applied to investigate percutaneous penetration profiles of drugs in this paper.Raman spectra of the ex vivo skin samples were collected by mapping technique.Then,a semiquantitative analysis model of concentration and signal intensity of an analyte had been established using multiple linear regression.On this basis,the contribution coefficients of concentration were presented in the form of heat maps to achieve the visual analysis of permeability distribution of the analyte in the skin.Percutaneous permeation analysis of salicylic acid in pig skins was conducted using Raman spectroscopy and imaging technology.The concentration contribution coefficients of salicylic acid were 0.231%and 0.275%in the stratum corneum,and 3.1×10^(–4) and 2.4×10^(–4)in the active epidermal layer in the parallel experiments.Compared with the results determined by HPLC method,it was found that the change trend of drug concentration by the two methods was consistent,indicating that the concentration contribution coefficient obtained by Raman spectroscopy could be used for semiquantitative analysis,which provided a new technical means for studying the percutaneous absorption characteristics of the analyte and formulation optimization.In addition,the permeation distribution of hyaluronic acid with different relative molecular weights in pig skin was also investigated by this method,and the visual analysis of distribution of macromolecular drugs in skin was also realized.