文献类型：期刊文献

英文题名：Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source

作者：Zou, X.[1,2];Chen, C. -F.[1];Hang, H. -F.[1];Chu, J.[1];Zhuang, Y. -P.[1];Zhang, S. -L.[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China

年份：2010

卷号：24

期号：1

起止页码：95

外文期刊名：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY

收录：;EI(收录号：20101412822187);WOS:【SCI-EXPANDED(收录号:WOS:000280574900013)】；

基金：This work was financially supported by the National High Technology Research and Development Program of China (863 Program), No.2006AA020304, and the Major State Basic Research Development Program of China (973 Program), No. 2007CB714303.

语种：英文

外文关键词：Biological nitrogen; erythromycin production; fed-batch strategy; medium optimization; response surface methodology; Saccharopolyspora etythraea

摘要：A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production. The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly. A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: gamma = 5.1 g L-1, biological nitrogen gamma = 5.96 g L-1 and soybean flour gamma = 24.17 g Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L-1 stirred bioreactor, respectively. It was found that pH control could serve as an effective control strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL(-1) at 190 h. This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative for economic erythromycin production on a large scale.