文献类型：期刊文献

英文题名：A noninvasive method for measuring the oxygen binding-releasing capacity of hemoglobin-loaded polymeric nanoparticles as oxygen carrier

作者：Zhang, Xiaolan[1];Liu, Changsheng[1];Yuan, Yuan[1];Shan, Xiaoqian[1];Sheng, Yan[1];Xu, Feng[1]

机构：[1]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Key Lab Ultrafine Mat Minist Educ, Shanghai 200237, Peoples R China

年份：2009

卷号：20

期号：5

起止页码：1025

外文期刊名：JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

收录：;EI(收录号：20091512030581);WOS:【SCI-EXPANDED(收录号:WOS:000264837700003)】；

基金：The authors appreciate the financial support from the National High Technology Research and Development Program of China ( 863 Program) ( No. 2004AA-302050) and from Shanghai Nanotechnology Special Foundation ( No. 0452nm022).

语种：英文

外文关键词：Biodegradable polymers - Noninvasive medical procedures - Nanoparticles - Infrared devices - Oxygen

摘要：Based on the strong penetration capacity of near infrared lights (NIRs) and different absorption of oxyhemoglobin and deoxyhemoglobin in NIRs region, a novel noninvasive method, with the aid of an airproof-equilibrium apparatus, was developed to determine the oxygen binding-releasing capacity, including oxygen dissociation curve (ODC) and P-50, of the hemoglobin-loaded polymeric nanoparticles (HbP) in this study. The measured ODC of the PLA-PEG HbP was very close to that of the native hemoglobin, and the corresponding P-50 (26.1 mmHg) was also near to the native precursor protein (27.3 mmHg), indicative of the validity of the method proposed. To further verify the method proposed, the oxygen binding-releasing capacity of the HbPs prepared by PCL, PCL-PEG, PLA were also investigated with human blood as control. These results indicated that the method developed here enabled accurate and noninvasive determination of the oxygen binding-releasing capacity of the biodegradable polymeric oxygen carriers.