文献类型：期刊文献

中文题名：纤维素酶蛋白质工程的新进展

英文题名：The Recent Advances in Protein Engineering of Cellulases

作者：赵喜华[1];涂宗财[1];魏东芝[2];王玮[2];唐平平[1]

机构：[1]江西师范大学生命科学学院,江西南昌330022;[2]华东理工大学生物工程学院,生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237

年份：2015

卷号：39

期号：4

起止页码：425

中文期刊名：江西师范大学学报（自然科学版）

外文期刊名：Journal of Jiangxi Normal University(Natural Science Edition)

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家自然科学基金(31360217);江西省教育厅自然科学基金(GJJ11071);江西师范大学博士科研启动基金(5451)资助项目

语种：中文

中文关键词：纤维素酶;蛋白质工程;新进展

外文关键词：cellulase; protein engineering; recent advances

摘要：原始纤维素酶通常存在催化活性低下、末端产物抑制、热稳定性不高、持续催化能力弱等问题,因此,改善它的酶学性质是很有意义的工作.目前,采用体外进化技术是改进纤维素酶酶学性质的一种有效途径.从纤维酶的催化域、结合域和连接区等不同区域出发,综述了纤维素酶蛋白质工程的新进展.提出了系统组装3个已改造功能结构域的思想,这有助于提高纤维素酶催化活性、持续催化能力、耐高浓度产物、热稳定性等特性,将促进纤维素酶在能源、工业、农业、食品等领域的高效应用.
Parent cellulases exist in some defects of a low catalytic activity,inhibition of end products,weak thermostability,and poor processivity,and therefore improving enzymatic properties of cellulases is a very meaningful job.To date,on the basis of the technology of evolution in vitro,it is an effective way to develop enzymatic properties of cellulases. Recent advances in protein engineering about three different regions of cellulases which consist of catalytic domains,binding domains and linker regions are reviewed in the paper. The idea about systematically assembling functional structure domains which have been engineered was put forward to in the paper,which will help to improve the enzymatic properties such as catalytic activity,the ability of processive hydrolysis,resistantance to high concentration of product,and thermalstability,and to promote high-efficent application in energy,industry,agriculture,food,and so on.