文献类型：期刊文献

中文题名：铁蛋白系统纳米粒子的构建及其相互作用

英文题名：Construction and Interaction of Ferritin Based System Nanoparticles

作者：沈阳[1];边成原[1];詹家荣[2];曹旭妮[1]

机构：[1]华东理工大学生物工程学院,上海200237;[2]上海化学试剂研究所有限公司,上海200333

年份：2018

卷号：44

期号：3

起止页码：308

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(21375039);上海市科委项目(12nm0502300;14JC1490902);生物反应器工程国家重点实验室项目(2060204)

语种：中文

中文关键词：系统纳米医学;铁蛋白;纳米粒子相互作用

外文关键词：systems nanomedicine;FTH1;nanoparticles interaction

摘要：以铁蛋白(FTH1)为基础,围绕系统纳米粒子的肿瘤靶向性、高载性和纳米粒子间的靶向性识别等关键点,构建了具有"靶向/聚集"特性的系统纳米粒子,并对其关键特性进行了详细研究。实验结果表明,"靶向纳米粒子"RGD/HSA-FTH1-biotin不仅具备靶向整合素αvβ3的特性,还能够高载生物素(Biotin)分子(纳米粒子与载有的生物素的物质的量之比为40);同时"聚集纳米粒子"mSA-FTH1/FTH1能特异性识别生物素并与之结合。这些研究显示了"靶向/聚集"纳米粒子的成功构建为该系统纳米粒子策略在微小肿瘤的诊断治疗方面的应用打下了重要基础。
Nanomedicine has been developed and applied for a long time. Although the rapid development of engineered nanoparticles with integration of different functions shows a great promise for the diagnosis and treatment of disease,nanoparticles responsible for in vivo targeted imaging of tiny tumors have not been available in clinic.Low sensitivity is one of the major problems.In this case,＂Systems nanomedicine＂has recently become an area of increased research focus.A pair of nanoparticles with signal amplification can be designed for this purpose.First,targeting nanoparticles are designed bearing agreat number of signaling molecules,while accumulating nanoparticles with fluorescence activity can specially bind to the signaling molecules on the surface of the targeting nanoparticles.With this pair of nanoparticles,signals for the imaging can be amplified because of large quantities of accumulating nanoparticles on the tiny tumors.Based on this principle,we constructed a pair of ferritins based targeting/accumulating nanoparticles to optimize the crucial technological issues including targeting ability,loading capacity and specific interaction between them.The results showed that RGD/HSAFTH1-biotin targeting nanoparticles could not only target and bind to integrinαvβ3,but also carry large amount of biotin moleculars（with a molar ratio 40）.At the same time,mSA-FTH1/FTH1 accumulating nanoparticles was found to be able to specifically bind to biotin.These results suggested a successful fabrication of the systems nanoparticles,which therefore laid a solid foundation for the diagnosis and treatment of tiny tumors in vivo.