文献类型：期刊文献

英文题名：Infection patterns, clinical significance, and genetic characteristics of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle in Jiangsu, China

作者：Wang, Rui[1];Li, Na[2];Jiang, Wen[1];Guo, Yaqiong[2];Wang, Xiaolan[1];Jin, Yue[1];Feng, Yaoyu[1,2];Xiao, Lihua[2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China

年份：2019

卷号：118

期号：10

起止页码：3053

外文期刊名：PARASITOLOGY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000487313500032)】；

基金：This work was supported in part by the National Natural Science Foundation of China (31602042) and National Key R&D Program of China (2017YFD0501305, 2017YFC1601206).

语种：英文

外文关键词：Enterocytozoon bieneusi; Giardia duodenalis; Dairy cattle; Transmission; Multilocus genotyping

摘要：The infection patterns and clinical significance of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle remain poorly investigated despite their common occurrence. Data on the genetic diversity are also needed to understand the transmission and human-infective potential of the two pathogens. In this study, fecal specimens from 1366 dairy cattle on a large farm were examined for the presence and genotype distribution of E. bieneusi and G. duodenalis by PCR and DNA sequencing. The overall infection rates of E. bieneusi and G. duodenalis were 13.0% and 20.6%, respectively. Pre-weaned calves had significantly higher infection rates of both pathogens than post-weaned and adult cattle (P < 0.001), with peak occurrence of the pathogens in animals of 7-12 weeks. In both pre- and post-weaned calves, animals with diarrhea were 2.1-3.0 times more likely to be infected with either pathogen than those without diarrhea (P < 0.01). The E. bieneusi identified belonged to five genotypes, including J (n = 138), I (n = 21), BEB4 (n = 10), Type IV (n = 1), and a novel genotype CHC17 (n = 1). Genotype J was the dominant one in all age groups, whereas genotype I was only identified in calves of 6-11 weeks. Genotyping of G. duodenalis at three genetic loci identified assemblage E (n = 278), assemblage A (n = 2), and concurrence of the two (n = 1). Altogether, 13, 7 and 10 subtypes of assemblage E were detected at the bg, gdh, and tpi loci, respectively, forming 65 multilocus genotypes. The formation of two major clusters of MLGs in eBURST analysis indicated that intra-assemblage genetic recombination of two dominant MLGs could have led to the high genetic heterogeneity within assemblage E on a single farm. Results of this study provide much needed data on the pathogenicity of E. bieneusi and G. duodenalis in pre- and post-weaned calves. The clinical significance of the two pathogens in dairy cattle warrants further investigations.