文献类型：期刊文献

英文题名：DNAzyme self-assembled gold nanoparticles for determination of metal ions using fluorescence anisotropy assay

作者：Yin, Bin-Cheng[1];Zuo, Peng[1];Huo, Hao[1];Zhong, Xinhua[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：401

期号：1

起止页码：47

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201908623127);WOS:【SCI-EXPANDED(收录号:WOS:000276978700007)】；

基金：This work was supported by grants of the National Science Foundation (NSF, 20627005, 20776039), SKLBE Fund (2060204), Shanghai Project (09JC1404100), NCET-07-0287, and Shuguang (06SG32).

语种：英文

外文关键词：DNAzyme; Gold nanoparticles; Copper; Lead; Fluorescence anisotropy

摘要：Gold nanoparticles can be exploited to facilitate a highly sensitive and selective metal ion detection based on fluorescence anisotropy assay with metal ion-dependent DNA-cleaving DNAzyme. This assay allows rapid and accurate determination of metal ions in aqueous medium at room temperature. The method has been demonstrated for determination of Cu2+ and Pb2+ ions. The detection sensitivity can be significantly improved to 1 nM by using a "nanoparticle enhancement" approach. Moreover, the assay was also tested in 384-well plates for high-throughput routine determination of toxic metal ions in environmental samples. The method showed distinct advantages over conventional methods in terms of its potential sensitivity, specificity, and ability for rapid response. (C) 2010 Elsevier Inc. All rights reserved.