文献类型：期刊文献

英文题名：A ratiometric hydrophilic fluorescent copolymer sensor based on benzimidazole chromophore for microbioreactors

作者：Shen, Liangjun[1]; Zhao, Ping[1]; Zhu, Weihong[1]

机构：[1] Key Laboratory for Advanced Materials, Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, China

年份：2011

卷号：89

期号：3

起止页码：236

外文期刊名：Dyes and Pigments

收录：EI(收录号：20105213536076)

基金：We thank the support from NSFC/China , the Program for New Century Excellent Talents in University ( NCET-06-0418 ), Shanghai Shuguang Project ( 07SG34 ), and Specialized Research Fund for the Doctoral Program of Higher Education ( SRFDP 200802510011 ).

语种：英文

外文关键词：Hydrophilicity - Photobleaching - pH sensors - Fluorescence - Bioreactors - pH - Chromophores

摘要：The ratiometric approach is robust and insensitive to factors such as source intensity, photobleaching, or orientation of the patch. A novel ratiometric hydrophilic copolymer of N-(1-ethyl-2-(pyridin-4-yl)-1H-benzo[d] imidazol-5-yl)methacrylamide and 2-hydroxyethyl methacrylate (P(BIPy-HEMA)) containing pyridyl substituted benzimidazole moiety as pH sensor has been developed. Its fluorescence in aqueous system showed two obvious isosbestic points due to two-stepwise protonation. According to the linear curves of I 462/I423 and I536/I462 to pH, such ratiometric pH values could be read directly without external calibration. Considering the hydrophilicity, stability and repeatability in aqueous environment, the fluorescent film with ratiometric characteristic over acidic pH range of 1.7-4.5 makes it high promising for online monitor in microbioreactors. ? 2010 Elsevier B.V. All rights reserved.