文献类型：期刊文献

英文题名：Lincomycin, rational selection of high producing strain and improved fermentation by amino acids supplementation

作者：Ye, Ruifang[1];Wang, Qian[1];Zhou, Xiaofang[1]

机构：[1]E China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China

年份：2009

卷号：32

期号：4

起止页码：521

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20092312107974);WOS:【SCI-EXPANDED(收录号:WOS:000266238900011)】；

语种：英文

外文关键词：Lincomycin; Rational screening; Propylproline; Amino acids; Metabolic engineering

摘要：Based on the report that the introduction of the biosynthetic precursor of lincomycin, propylproline, could increase the production of lincomycin (Bruce et al. in US Patent 3,753,859, 1973), a mutant strain pro10-20, with resistance of feedback suppression of proline (an analog of propylproline) was thus selected and lincomycin production increased by 10%. The addition of three amino acids (l-proline, l-tyrosine, l-alanine) which are the precursors of propylproline to the fermentation medium was found to enhance the accumulation of l-dopa through different pathways and was favorable to lincomycin biosynthesis. The production of lincomycin was increased by 23, 10, 13%, respectively, with the addition of 0.05 g L-1 l-proline at 60 h, 0.005 g L-1 l-tyrosine and 0.1 g L-1 l-alanine directly in the medium.