文献类型：期刊文献

英文题名：Production and characterization of a C₁₅-surfactin-O-methyl ester by a lipopeptide producing strain Bacillus subtilis HSO121

作者：Liu, Xiang-Yang;Yang, Shi-Zhong;Mu, Bo-Zhong[1]

机构：[1]E China Univ Sci & Technol, Adv Mat Lab, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2009

卷号：44

期号：10

起止页码：1144

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000270259100013)】；

基金：This study was supported by the National Natural Science Foundation of China (No. 50574040), the grant from the Ministry of Science and Technology of China (2007CB707801) and from Shanghai Science and Technology Commission (No. 071607014).

语种：英文

外文关键词：Surfactin; Bacillus subtilis; Structure; Surface activity; Hela cell; Carboxyl group

摘要：A surfactin mono-methyl ester was isolated from the cell broth of Bacillus subtilis HSO121 by acid precipitation, methanol extraction and two-step chromatographic methods. The structure was analyzed by GC/MS, HPLC, ESI Q-TOF MS/MS and NMR. It indicates that this ester is a C-15-surfactin-O-methyl ester of which the fatty acid portions are composed of iso-C-15 and anteiso-C-15 beta-hydroxy fatty acids. Based on the critical micelle concentration (CMC) in phosphate buffered saline (PBS, pH 8.0) and the IC50 value on Hela cell lines, the C-15-surfactin-O-methyl ester has a stronger surface activity and lower antitumoral effect than C-15-surfactin does. It is found that the Glu residues of surfactin-like lipopeptides play a role in their anti-proliferative effects on Hela cells. The possible antitumoral activity of surfactin is discussed in relation to their structures. (C) 2009 Elsevier Ltd. All rights reserved.