文献类型：期刊文献

英文题名：The effects of angiotensin I-converting enzyme inhibitory peptide VGINYW and the hydrolysate of α-lactalbumin on blood pressure, oxidative stress and gut microbiota of spontaneously hypertensive rats

作者：Xie, Dewei[1,2];Shen, Yaling[1,2];Su, Erzheng[3];Du, Lei[1,2];Xie, Jingli[1,2,4];Wei, Dongzhi[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Dept Food Sci & Technol, Shanghai 200237, Peoples R China;[3]Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China

年份：2022

卷号：13

期号：5

起止页码：2743

外文期刊名：FOOD & FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000755919100001)】；

基金：This work was supported by the National Key Research and Development Program of China (no. 2020YFA0907800), China; the National Natural Science Foundation of China (grant no. 31801489); and the Natural Science Foundation of Shanghai (no. 21ZR1416200).

语种：英文

摘要：VGINYW is a highly active angiotensin I-converting enzyme (ACE) inhibitory peptide discovered from alpha-lactalbumin by an in vitro-in silico high throughput screening strategy. The aim of this study was to evaluate the antihypertensive effect of the peptide and the alpha-lactalbumin hydrolysates under 3 kDa (LH-3k), and illustrate the possible mechanism in spontaneously hypertensive rats (SHRs). SHRs were administered with VGINYW and LH-3k at doses of 5 mg per kg BW and 100 mg per kg BW, respectively. VGINYW and LH-3k could markedly decrease the systolic blood pressure (SBP) of the SHRs, and the maximal drops of 21 mmHg (2 h after administration) and 17 mmHg (4 h after administration) were achieved during the 8 hour test, respectively. When the agents were given once per day for 4 weeks, they caused a long-term decrease of 16 mmHg of SBP. VGINYW and LH-3k control the blood pressure through regulating the renin-angiotensin system by inhibiting the ACE activity and diminishing the angiotensin II level, and further upregulating the expression levels of the angiotensin-converting enzyme 2 and angiotensin type 2 receptor, and downregulating the expression of the angiotensin type 1 receptor. VGINYW and LH-3k could notably ameliorate the oxidative stress in the SHR as well. It is more important that the gavage of VGINYW and LH-3k could alleviate hypertension-associated intestinal microbiota dysbiosis by recovering the diversity of the gut microbiota and altering the key floras which are short chain fatty acid producers. In conclusion, VGINYW and LH-3k are effective functional ingredients for blood pressure control.