文献类型：期刊文献

英文题名：Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide

作者：Ma, Zheyong[1];Yi, Xiaoping[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：43

期号：6

起止页码：690

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20081911243211);WOS:【SCI-EXPANDED(收录号:WOS:000256569600014)】；

语种：英文

外文关键词：cell culture; recombinant CHO cell; dimethyl sulfoxide; HBsAg; sucrose gradient analysis; intracellular accumulation

摘要：The intracellular hepatitis B surface antigen (HBsAg) content per cell was significantly increased by 7.2-fold in the culture of recombinant CHO cells with 1.5% dimethyl sulfoxide (DMSO), while the HBsAg production and specific productivity were only improved by 70% and 3.2-fold, respectively. The significant accumulation of HBsAg within rCHO cells by DMSO stimulation was testified with flow cytometry measurements. Electron microscopy was applied to show that the dilated areas scattered over whole cytoplasm within rCHO cells in response to DMSO, and further revealed that intracellular HBsAg was localized to these areas with immunogold labeling. The failure of intracellular HBsAg virus-like particle assembly was revealed to be closely associated with the HBsAg accumulation within DMSO-stimulated rCHO cells on the basis of sucrose gradient analysis of cell extract. This work provided the details to further understand the HBsAg accumulation within rCHO cells in response to DMSO. (C) 2008 Elsevier Ltd. All rights reserved.