文献类型：期刊文献

英文题名：Cytotoxic effects of Avermectin on human HepG2 cells in vitro bioassays

作者：Zhang, Yang[1];Wu, Jigang[1];Xu, Wenping[1];Gao, Jufang[2];Cao, Haijing[1];Yang, Mingjun[1];Wang, Bo[1];Hao, Youwu[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China

年份：2017

卷号：220

起止页码：1127

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;EI(收录号：20164903093680);WOS:【SCI-EXPANDED(收录号:WOS:000390732300041)】；

基金：This work was financial supported by National Key Technology Research Development Program of China (NO.2011BAE06B04).

语种：英文

外文关键词：Environmental toxicity; Pesticide; Avermectin; HepG2 cells; Cytotoxic effects; Mitochondrial-mediated apoptosis

摘要：Avermectin (AVM) has been widely used in agriculture and animal husbandry based on its broad spectrum of effective anthelmintic activity and specificity targets. However, AVM induction of cytotoxicity in human liver is largely unknown. In this study, we investigate the cytotoxic effects of AVM on HepG2 cells in vitro. The results revealed that AVM inhibited the viability of HepG2 cells and enhanced apoptosis. Established assays of cytotoxicity were performed to characterize the mechanism of AVM toxicity on HepG2 cells. Typical apoptosis morphological changes were shown in AVM-treatment cells including chromatin condensation and DNA fragmentation. We demonstrated that AVM-induced apoptosis of HepG2 cells were mediated by generated ROS. Moreover, a decrease in mitochondrial membrane potential (MMP) and up-regulating the Bax/Bcl-2 ratio, resulted in a release of cytochrome-c as well as activation of caspase-9/-3. In conclusion, our experimental results show that AVM has a potential threat to human health which may be induce apoptosis of human hepatocyte cells via caspase-dependent mitochondrial pathways. (C) 2016 Elsevier Ltd. All rights reserved.