文献类型：期刊文献

英文题名：Impedimetric biosensor fabricated with affinity peptides for sensitive detection of Escherichia coli O157:H7

作者：Shi, Fan[1];Gan, Lingfeng[1];Wang, Yibing[1];Wang, Ping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg, Biomed Nanotechnol Ctr,Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2020

卷号：42

期号：5

起止页码：825

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20200508101709);WOS:【SCI-EXPANDED(收录号:WOS:000515632400002)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos.31471659, 21636003 and 21303050) and the Natural Science Foundation of Shanghai (Grant No.19ZR1412400).

语种：英文

外文关键词：E. coli O157; H7; Affinity peptides; Biosensor; Electrochemical impedance spectroscopy

摘要：Objective To effectively and conveniently detect pathogenic bacteria, this study aimed to develop label-free biosensors fabricated affinity peptides that can recognize targeted bacteria strains and enable precise quantitative detections. Results A 12-mer peptide with high binding affinity toward Escherichia coli O157:H7 was discovered by biopanning of phage-displayed peptide library. The peptide modified with glycine residues (G(3)) and one cysteine (C) residue at C-terminal, could self-assemble on gold electrodes, enabling electrochemical impedance spectroscopy (EIS) analysis for quantitative detection of E. coli O157:H7. This method showed a low detection limit of 20 CFU/mL and a liner range from 2 x 10(2) to 2 x 10(6) CFU/mL. Conclusion It appears that, by designing and optimizing the structures of peptides, such a strategy can be greatly promising in developing quick, sensitive and quantitative biosensor of pathogens.