文献类型：期刊文献

中文题名：高效液相色谱-串联质谱法分析姜黄中微量的姜黄素类化合物

英文题名：Analysis of minor curcuminoids in Curcuma longa L. by high performance liquid chromatography-tandem mass spectrometry

作者：李伟[1];肖红斌[1];王龙星[1];梁鑫淼[1,2]

机构：[1]中国科学院大连化学物理研究所,辽宁大连116023;[2]华东理工大学药学院,上海200237

年份：2009

卷号：27

期号：3

起止页码：264

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：CSTPCD;;Scopus;WOS:【ESCI(收录号:WOS:000435241000003)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家杰出青年科学基金项目(20825518);科技部国际合作项目(2007DFC30550);辽宁省科技计划重大项目(2006226002);大连市科技计划项目(2007J23JH032)

语种：中文

中文关键词：高效液相色谱-串联质谱法;多反应监测模式;姜黄素类化合物;微量成分;姜黄

外文关键词：high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/ MS） ; multi-reaction monitoring （MRM） mode; curcuminoids; minor constituents; Curcuma longa L.

摘要：建立了同时检测中药姜黄中3种微量的姜黄素类化合物的高效液相色谱-电喷雾串联质谱分析方法。姜黄根茎经乙醇超声提取后,无需其他处理可直接进样分析。以Microsorb C18色谱柱(250mm×4.6mm,5μm)分离,乙腈和0.1%甲酸水梯度洗脱,在多反应监测模式(MRM)下对目标成分进行定性分析。利用质谱碎裂规律,分别对每个目标成分同时监测8个母离子/特征子离子对的反应过程,首次从姜黄中发现了3个微量的姜黄素类化合物成分。一次性完成了目标成分的同时定性,方法的检出限为0.2μg/L。结果表明,该方法具有简便、快速、准确、灵敏度高的优点,适用于中药复杂体系中姜黄素类化合物的分析检测。
A high performance liquid chromatography-tandem mass spectrometric method has been developed for the simultaneous analysis of three minor curcuminoids in the crude extract of Curcuma longa L. After ultrasonic extraction with ethanol, the sample was separated on a Microsorb C18 column （250 mm×4.6 mm, 5 μm） with the gradient elution of acetonitrile and 0.1% （v/v） formic acid aqueous solution as mobile phase, then detected by electrospray ionization tandem mass spectrometry in multi-reaction monitoring （MRM） mode. On the basis of analysis of the fragmentation patterns, the identification of the minor ones was achieved in a single experiment using MRM with 8 precursor-product ion transitions for each component. Using this technique, the three minor curcuminoids were observed in C. longa L. for first time and the limit of detection was 0.2 μg/L. The experimental results show that this method is simple, rapid, accurate, sensitive and suitable for the analysis of curcuminoids in the complex samples of traditional Chinese medicine.