文献类型：期刊文献

英文题名：A Facile and Versatile Approach to Construct Photoactivated Peptide Hydrogels by Regulating Electrostatic Repulsion

作者：Xiang, Yanxin[1,2];Mao, Huanv[1,2];Tong, Si-cheng[3];Liu, Can[1,2];Yan, Rui[1,2];Zhao, Li[3];Zhu, Linyong[1,2,4];Bao, Chunyan[1,2,4]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem,Sch Che, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem,Sch Che, Shanghai 200237, Peoples R China;[3]Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：17

期号：6

起止页码：5536

外文期刊名：ACS NANO

收录：;EI(收录号：20231113710435);WOS:【SCI-EXPANDED(收录号:WOS:000948753300001)】；

基金：This work was supported by Shanghai Sci. Tech. Comm. (21ZR1415500) , National Key Research and Development Project (2019YFA0110500) , and Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 0328) . We thank the Research Centre of Analysis and Test of East China University of Science and Technology for the help with the characterization.

语种：英文

外文关键词：peptide hydrogel; electrostatic repulsion; photoactivation; spatiotemporal control; cell culture

摘要：Short peptides that can respond to external stimuli have been considered as the preferred building blocks to construct hydrogels for biomedical applications. In particular, photoresponsive peptides that are capable of triggering the formation of hydrogels upon light irradiation allow the properties of hydrogels to be changed remotely by precise and localized actuation. Here, we used the photo-chemical reaction of the 2-nitrobenzyl ester group (NB) to develop a facile and versatile strategy for constructing photoactivated peptide hydrogels. The peptides with high aggregation propensity were designed as hydrogelators, which were photocaged by a positively charged dipeptide (KK) to provide strong charge repulsion and prevent self-assembly in water. Light irradiation led to the removal of KK and triggered the self-assembly of peptides and the formation of hydrogel. Light stimulation endows spatial and temporal control, which enables the formation of hydrogel with precisely tunable structure and mechanical properties. Cell culture and behavior study indicated that the optimized photoactivated hydrogel was suitable for 2D and 3D cell culture, and its photocontrollable mechanical strength could regulate the spreading of stem cells on its surface. Therefore, our strategy provides an alternative way to construct photoactivated peptide hydrogels with wide applications in biomedical areas.