文献类型：期刊文献

英文题名：pH shifting adaptive evolution stimulates the low pH tolerance of Pediococcus acidilactici and high L-lactic acid fermentation efficiency

作者：Li, Zhibin[1];Zhang, Lingxiao[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：416

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20244717377872);WOS:【SCI-EXPANDED(收录号:WOS:001359836100001)】；

基金：This research was supported by the National Key R&D Program of China (2023YFA0914400), the National Natural Science Foundation of China (32301269), the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900).

语种：英文

外文关键词：Adaptive evolution; pH shifting; Lactic acid bacteria; Cell viability; Wheat straw

摘要：L-lactic acid fermentation at low pH reduces the use of neutralizers during fermentation and the generation of solid wastes in purification processes. Most lactic acid bacteria exhibit weak tolerance and poor cell viability at low pH. This study proposes a pH shifting adaptive evolution method to improve the low-pH tolerance of an engineered Pediococcus acidilactici strain. In the first stage, cells were cultured at a moderate pH to maintain the cell viability, then shifted to a low pH to enhance low-pH tolerance. Long-term pH shifting evolution culture of the engineered P. acidilactici between the moderate and low pH resulted in a 43 % increase in L-lactic acid production at pH 4.6 (110.4 g/L) and a 2.1-fold increase at pH 4.4 (80.7 g/L) compared to the parental strain when using wheat straw as a feedstock. This pH-shifting adaptive evolution strategy provides an effective tool for improving the low-pH tolerance of lactic acid bacteria.