文献类型：期刊文献

英文题名：Adsorption characteristics and preparative separation of chaetominine from Aspergillus fumigatus mycelia by macroporous resin

作者：Liu, Changqing[1,2];Jiao, Ruihua[3];Yao, Lingyun[1,2];Zhang, Yupeng[1,2];Lu, Yanhua[1,2];Tan, Renxiang[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Box 283,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Inst Funct Biomol, Nanjing 210093, Jiangsu, Peoples R China

年份：2016

卷号：1015

起止页码：135

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20161002045358);WOS:【SCI-EXPANDED(收录号:WOS:000372685500014)】；

基金：This work is financially supported by the National High Technology Research and Development Program of China (2013AA092901), the Fundamental Research Funds for the Central Universities, as well as the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：Adsorption kinetics and isotherms; Aspergillus fumigates; Chaetominine Macroporous adsorption resin; Separation

摘要：Chaetominine (CHA) is a quinazolinone alkaloid with strong anti-cancer activity produced by Aspergillus fumigatus CY018. For recovering CHA from A. fumigates efficiently, adsorption and desorption capacities of eight macroporous resins were tested in this work. Based on batch experiments, XAD-16 resin was revealed the best adsorption and desorption performance among all the tested resins. Then, adsorption kinetics and adsorption isotherms were constructed on XAD-16 resin, and the experimental data were fitted well to the pseudo first-order kinetics and Freundlick isotherm model. In the dynamic adsorption and desorption, the purity of CHA increased from 0.0314% (w/w) in the crude extract to 57.86% in the final product with recovery yield of 70.56% by a one-step treatment. Moreover, the experiments were also performed in a lab scale-up scale, in which the purity and recovery of CHA were 56.12% (w/w) and 68.02%, respectively. In addition, XAD-16 resin could be recycled 3 times for CHA separation after regeneration without adverse effects on adsorption/desorption performance. These results suggested that XAD-16 resin adsorption could act as a useful and economic method for recovering CHA from A. fumigatus. (C) 2016 Elsevier B.V. All rights reserved.