文献类型：期刊文献

英文题名：Inactivation and augmentation of the primary 3-Ketosteroid- δ1-Dehydrogenase in mycobacterium neoaurum NwIB-01: Biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione

作者：Wei, Wei[1]; Wang, Feng-Qing[1]; Fan, Shu-Yue[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

年份：2010

卷号：76

期号：13

起止页码：4578

外文期刊名：Applied and Environmental Microbiology

收录：EI(收录号：20102713062613)

语种：英文

外文关键词：Gene encoding - Polyols

摘要：3-Ketosteroid-δl-dehydrogenase, KsdDM, was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from l,4-androstadiene-3,17-dione (ADD) is a key bottleneck to the microbial transformation of phytosterols in industry. This problem was tackled via genetic manipulation of the KsdD-encoding gene. Mutants in which KsdDM was inactivated or augmented proved to be good AD (D)-producing strains. Copyright ? 2010, American Society for Microbiology. All Rights Reserved.