文献类型：期刊文献

英文题名：Ionizable mesoporous silica nanoparticle-mediated siAURKB delivery for colorectal cancer therapy

作者：Yao, Yuhao[1];Leng, Yuheng[1];Li, Xilong[1];Chen, Pengcheng[2];Zou, Jinglin[1];Shi, Lei[2];Zhang, Dapeng[1];Li, Yongsheng[1]

机构：[1]East China Univ Sci & Technol, Frontier Sci Ctr Mat Biol & Dynam Chem, Sch Mat Sci & Engn,Lab Low Dimens Mat Chem, Key Lab Ultrafine Mat Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Univ Chinese Tradit Med, Shuguang Hosp, Dept Clin Lab, Shanghai 201203, Peoples R China

年份：2026

卷号：14

期号：11

起止页码：3573

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20261020214162);WOS:【SCI-EXPANDED(收录号:WOS:001706716000001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (Grant No. 2024YFF0508600), the National Natural Science Foundation of China (No. 22305081, 32571562, 52572303), Leading Talents in Shanghai in 2018, Shanghai Sailing Program (23YF1408600), the 111 project (B14018), and the Fundamental Research Funds for the Central Universities of China. We thank the staff members of the Integrated Laser Microscopy System at the National Facility for Protein Science in Shanghai for providing technical support and assistance in data collection and analysis.

语种：英文

外文关键词：Cell death - Controlled drug delivery - Diseases - Mammals - Medical nanotechnology - Nanoparticles - Oncology - RNA - Silica - Surface treatment - Targeted drug delivery - Tumors

摘要：Colorectal cancer (CRC) is a common malignant tumor with high incidence and mortality worldwide. Conventional therapeutic strategies, such as surgical resection and chemotherapy, which result in improved outcomes for some patients, are greatly hindered by drug resistance and off-target toxicity. Small interfering RNA (siRNA)-mediated RNA interference has provided a breakthrough for precision CRC therapy. However, efficient delivery of siRNA to the tumor site with its complex tumor microenvironment remains challenging. Here, an ionizable mesoporous silica nanoparticle (MSN)-based nanocarrier with high siRNA loading capacity and enhanced cell membrane penetration capability is developed for effective CRC therapy. By modifying diethylamino groups onto the surface of MSNs featuring maze-like pores, a new siRNA delivery carrier, denoted as MPMNC, is successfully prepared. MPMNC exhibits improved cellular uptake efficiency in CT26 cells and a significant fluorescence silencing effect in EGFP-293T cells. Furthermore, aurora-B siRNA (siAURKB), which downregulates the expression of AURKB in tumor cells to induce apoptosis, is loaded onto MPMNC to afford the nanomedicine siAURKB@MPMNC, which delivers siAURKB to tumor cells and inhibits tumor growth effectively (tumor growth inhibition rate of 64.9%) through intratumor injection in CRC model mice. Additionally, it exhibits excellent biocompatibility in vitro and in vivo. Overall, this work presents a novel nanomedicine, siAURKB-loaded MSN, highlighting its surface modification with ionizable amino groups, which offers a promising therapeutic paradigm for CRC.