文献类型：期刊文献

英文题名：A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris

作者：Zhang, Jianguo[1];Wang, Xuedong[1];Su, Erzheng[1];Fang, Guochen[1];Ren, Yuhong[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, Coll Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：41

期号：1

起止页码：74

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257906200012)】；

语种：英文

外文关键词：high cell density fermentation; methanol consumption rate; S-adenosylmethionine; Pichia postoris

摘要：A recombinant Pichia pastoris MutS expressing SAM2 gene ofSaccharomyces cerevisiae was cultured for Sadenosylmethionine (SAM) accumulation. Effect of the amount of methanol added (0.5%J0%,2.0%,3.0%, 4.0%,6.0%, 10.0%, and 12.0%) and cell densities (9.57,13.47,21.74,30.90, and 41.24 g/Ldry cell weight (DCW)) on yield of SAM was found in flask cultivations. In flask experiments, maximal yield of SAM (1.29 g/Q was obtained at 2.0% methanol added and 30.90 g/L DCW which gave the maximal methanol consumption rate. Conjunct effect of amount of methanol added and cell density was found through Origin 7.0 (7.0 Microcal, USA). Scale up in 3.7 L bioreactor, 51 % specific yield of SAM was enhanced at 0.6% methanol compared to that of 0.1 % methanol. In fed-batches of different cell densities at 0.6% methanol, maximal yield of SAM was 8.66 g/L at 100 g/L DCW with 64% yield of SAM enhanced again. Methanol consumption rate at 100 g/L DCW was 4.81 mL/L h. Maintenance coefficient of 100 g/L DCW was lower than that ofothers significantly, although methanol consumption rate of 90 g/L DCW was higher (5.07 mL/L h) than that of 100 g/L DCW. (c) 2008 Published by Elsevier B.V.