文献类型：期刊文献

英文题名：Switching the regioselectivity of two nitrilases toward succinonitrile by mutating the active center pocket key residues through a semi-rational engineering

作者：Chen, Zhi[1];Jiang, Shuiqing[1];Wang, Hualei[1];Wang, Liuzhu[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：55

期号：20

起止页码：2948

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000462657900012)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21676090), China Postdoctoral Science Foundation (No. 2017M621390), and the Fundamental Research Funds for the Central Universities (No. 222201814037).

语种：英文

摘要：The 163Ala in bll6402NIT and 172Trp in blr3397NIT played a significant role in directing nitrilase regioselectivity. Amino acid mutation of two nitrilases at these two sites could alter the size of the active site pocket and further switch nitrilase regioselectivity toward succinonitrile, which lays the foundation for subsequent research on regioselective nitrilase towards several dinitriles.