文献类型：期刊文献

英文题名：miR-139-5p Inhibits the Epithelial-Mesenchymal Transition and Enhances the Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating BCL2

作者：Li, Qingguo[1,2];Liang, Xin[3,4];Wang, Yuwei[1,2];Meng, Xianke[1,2];Xu, Ye[1,2];Cai, Sanjun[1,2];Wang, Zhimin[5,6];Liu, Jianwen[3,4];Cai, Guoxiang[1,2]

机构：[1]Fudan Univ, Shanghai Canc Ctr, Dept Colorectal Surg, Shanghai 200032, Peoples R China;[2]Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai 200032, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[5]Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Hlth & Dis Genom, Dept Genet, Shanghai 201203, Peoples R China;[6]Shanghai Acad Sci & Technol, Shanghai 201203, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000376885200001)】；

基金：This research was supported by the National Science Foundation of China (No. 81372646, 81101586, 81572351), the Laboratory Animal Program of Science and Technology Commission of Shanghai Municipality (No. 14140902200, 14140902201), and the National Key Basic Research Program of China (2014CBA02002). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Professor Qi Li in the Department of Oncology, Shanghai Shuguang Hospital affiliated with Shanghai University of T.C.M for providing NCM460 cells.

语种：英文

摘要：MicroRNAs (miRNAs) are important regulators involved in various cancers, including colorectal cancer (CRC). The functions and mechanisms of the miRNAs involved in CRC progress and metastasis are largely unknown. In this study, miRNA microarray analysis was performed to screen crucial miRNAs involved in CRC progress, and miR-139-5p was chosen for further study. The functional roles of miR-139-5p in colon cancer were demonstrated by CCK-8 proliferation assay, cell invasion and migration, cell apoptosis and in a KO mouse study. miR-139-5p expression was significantly decreased in cancer tissues compared to normal tissues. The miR-139-5p expression level was associated with tumour stage (P < 0.01). Function studies revealed that miR-139-5p was significantly correlated with the metastasis potential and drug resistance of colon cancer cells by affecting the epithelial-mesenchymal transition (EMT). Then, we identified BCL2 as a direct target of miR-139-5p cells in vitro. The patient samples and KO mice model showed that BCL2 expression was inversely correlated with the expression of miR-139-5p. In conclusion, we found that miR-139-5p targeted the BCL2 pathway to reduce tumour metastasis and drug sensitivity in CRC. This axis provided insight into the mechanism underlying miRNA regulation of CRC metastasis and a novel therapeutic target for CRC therapy.