文献类型：期刊文献

英文题名：Evaluation of Methanol Induced Free Radicals in Mice Liver

作者：Zhou, Lifang[1,2];Zhao, Hongli[1];Pan, Tieying[2];Trinchi, Adrian[3];Lan, Minbo[1,2];Wei, Gang[1,4]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]CSIRO Mfg, Private Bag 10, Clayton, Vic 3169, Australia;[4]CSIRO Mfg, POB 218, Lindfield, NSW 2070, Australia

年份：2017

卷号：70

期号：5

起止页码：499

外文期刊名：AUSTRALIAN JOURNAL OF CHEMISTRY

收录：;EI(收录号：20171903643008);WOS:【SCI-EXPANDED(收录号:WOS:000400667000009)】；

语种：英文

外文关键词：Mammals - Methanol - Electron spin resonance spectroscopy - Plants (botany) - Paramagnetic resonance - Superoxide dismutase

摘要：Methanol induced oxidative stress (OS) models in mice were successfully established and evaluated by the electron paramagnetic resonance (EPR) spin trapping technique. The capacity for removal of reactive oxygen species (ROS) free radicals by rhubarb and vitamin C (Vc) as candidate materials was also investigated. EPR was employed to determine the free radicals generated from a spin trapping agent, alpha-phenyl-N-tert-butylnitrone (PBN), that reacted with the ROS. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and the level of malondialdehyde (MDA) were also evaluated by enzyme assays. The results indicated that methanol clearly promoted the generation of ROS free radicals in the liver of mice. The activity of SOD and GSH-PX was reduced significantly, although the level of MDA was increased as a result of the harmful effect of methanol. In addition, rhubarb and Vc exhibited a protective effect on the mice liver under acute OS.