文献类型：期刊文献

英文题名：Fingerprint lipolytic enzymes with chromogenic p-nitrophenyl esters of structurally diverse carboxylic acids

作者：Qian, Le[1]; Liu, Jia-Yan[1]; Liu, Jia-Ying[1]; Yu, Hui-Lei[1]; Li, Chun-Xiu[1]; Xu, Jian-He[1]

机构：[1] Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2011

卷号：73

期号：1-4

起止页码：22

外文期刊名：Journal of Molecular Catalysis B: Enzymatic

收录：EI(收录号：20113914363670)

语种：英文

外文关键词：Data mining - Substrates - Lipases

摘要：A series of structurally diverse chromogenic esters, including a new compound (4-nitrophenyl 2-methylpentanoate), has been synthesized, constituting an array of 17 substrates which could be applied to rapidly fingerprint the activity of lipases or esterases to reveal their substrates specificity and functional characteristics. Combined with genetic technology such as "data mining" and directed evolution, such fingerprints might be a promising platform for discovery of potentially useful enzymes in industrial application. The fingerprint of commercially available Lipase-B from Candida antarctica as a model enzyme was first measured to confirm the reliability of this method. Then three new enzymes mined from genomic libraries were successfully fingerprinted, revealing the functional characteristics of those enzymes. Among them, the enzyme SrfAD was founded with specific substrate preference towards cycloalkyl carboxylic esters and aromatic esters, making it more promising in synthetic utilities than other tested enzymes. ? 2011 Elsevier B.V. All Rights Reserved.