文献类型：期刊文献

英文题名：A novel immobilization strategy using oligonucleotide as linker for small molecule microarrays construction

作者：Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Dept Food Engn Sci, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2008

卷号：23

期号：11

起止页码：1694

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20081911239283);WOS:【SCI-EXPANDED(收录号:WOS:000256491800017)】；

语种：英文

外文关键词：small molecule microarrays; immobilization; oligonucleotide; linker

摘要：A novel immobilization method based on oligonucleotide as linker has been developed for small molecule microarrays (SMMs) construction. The oligonucleotide tail was employed as a linker in solid-phase synthesis. Small molecules could be easily conjugated at the 5' end of the oligonucleotide, previously modified with a functional group. Being a reactive species, the oligonucleotide was activated by UV irradiation, for the attachment of the conjugate to the slide surface. The method was successfully applied to structurally distinct small molecules, including biotin, antibiotic and drug. This immobilization strategy showed high efficiency, 1.1 fmol of small molecules in the spotting solution per spot gave a detectable signal (mean S/N = 10.9). The results suggest that it is very promising for exploring interaction between small molecules and proteins, and high throughput detecting the chemical compounds. (c) 2008 Elsevier B.V. All rights reserved.