文献类型：期刊文献

英文题名：An Allosteric Dual-DNAzyme Unimolecular Probe for Colorimetric Detection of Copper(II)

作者：Yin, Bin-Cheng[1];Ye, Bang-Ce[1];Tan, Weihong[2,3];Wang, Hui[2,3];Xie, Cong-Cong[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Florida, Dept Chem & Physiol, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA;[3]Univ Florida, Shands Canc Ctr, Gainesville, FL 32611 USA

年份：2009

卷号：131

期号：41

起止页码：14624

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20094312400236);WOS:【SCI-EXPANDED(收录号:WOS:000271271800013)】；

基金：This work was supported by Grant 20627005 from the National Natural Science Foundation, the National Special Fund for SKLBE (2060204). NCET-07-0287 from the Program for New Century Excellent Talents, 09JC1404100 and 06SG32 from the Shanghai Shuguang Program, and U S NIH grants.

语种：英文

外文关键词：Nucleic acids - Signal transduction - Potable water - Colorimetry - Copper compounds

摘要：An effective dual-DNAzyme-based unimolecular probe design employing intramolecular signal transduction is demonstrated. The probe is composed of three domains: a DNA-cleaving DNAzyme, a substrate, and an HRP-mimicking DNAzyme. When the probe meets its target, cleavage of the substrate by the DNA-cleaving DNAzyme activates the HRP-mimicking DNAzyme, producing a colorimetric signal. The Cu2+-dependent DNAzyme engineered to demonstrate this design revealed a sensitivity corresponding to 65 ppb, which is sufficient to detect Cu2+ in drinking water. The new probe has excellent selectivity toward Cu2+. This three-component design is simple and easy to engineer. It may provide the basis for future development of other nucleic acid-based probes for toxicological and environmental monitoring.