文献类型：期刊文献

英文题名：Removal of antibiotic resistance genes and control of horizontal transfer risk by UV, chlorination and UV/chlorination treatments of drinking water

作者：Zhang, Tianyang[1,2];Hu, Yaru[1];Jiang, Lei[3];Yao, Shijie[1];Lin, Kuangfei[1];Zhou, Yanbo[1,2];Cui, Changzheng[1,2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[3]Natl Engn Res Ctr Urban Water Resources, Shanghai 200082, Peoples R China

年份：2019

卷号：358

起止页码：589

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20184105937136);WOS:【SCI-EXPANDED(收录号:WOS:000450105700056)】；

基金：This work was sponsored by Natural Science Foundation of China (No. 51808222), Shanghai Sailing Program (No. 18YF1406000), China Postdoctoral Science Foundation (No. 2017M621391), the National Water Pollution Control and Management Technology Major Projects (No. 2017ZX07402003), the Fundamental Research Funds for the Central Universities (No. 222201814055) and Shanghai Municipal Science and Technology Commission (No. 16DZ1204703).

语种：英文

外文关键词：Antibiotic resistant bacteria (ARBs); Antibiotic resistance genes (ARGs); Hydroxyl radicals (center dot OH); Reactive chlorine species (RCS); UV/chlorination

摘要：This study investigated the reduction of one antibiotic resistance gene (ARG, sul1) and one integron (intI1) within a multiple-antibiotic-resistant bacterium (ARB), Pseudomonas. HLS-6, during UV, chlorination and UV/chlorination. This bacterial strain was easily inactivated by these three methods, but its gene inactivation was not so easy. Two short target gene sequences (sul1-qPCR and intI1-qPCR) were selected for quantitative analysis, and another two longer ones (sul1-PCR and intI1-PCR) were used for qualitative identification. During the initial reaction time (<20 min), the degradation rate order of sul1-qPCR and intI1-qPCR was as follows: UV/chlorination>chlorination>UV. The log reduction of sul1-qPCR and intI1-qPCR in UV/chlorination achieved >3.50 and 4.00 log, respectively. The removal efficiency of sul1-PCR and intI1-PCR was also identified by gel electrophoresis analysis, which further confirmed the advantage of UV/chlorination treatment on DNA damage. Among the radicals produced in UV/chlorination, only the reactive chlorine species (Cl center dot, Cl-2 center dot(-) and ClO center dot) can degrade the target genes. Under the condition of low chlorine dosage, sul1-qPCR was easier to be removed than intI1-qPCR by UV/chlorination. The log reduction of both sul1-qPCR and intI1-qPCR decreased with pH increasing during UV/chlorination. In the presence of sulfamethoxazole, UV/chlorination showed more advantages than direct UV and chlorination in the simultaneous removal of antibiotics, ARBs and ARGs.