文献类型：期刊文献

中文题名：Effect of catalase on CPC production during fermentation of Acremonium chrysogenum

作者：Ling Liu[1];Zhen Chen[1];Xiwei Tian[1];Ju Chu[1]

机构：[1]Qingdao Innovation Institute of East China University of Science and Technology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road,Shanghai 200237,People’s Republic of China

年份：2025

卷号：12

期号：1

起止页码：1

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the National Key Research Development Program of China(2022YFC2105403);the Taishan Scholars Program,the Shanghai Pilot Program for Basic Research(22TQ1400100-14);the Natural Science Foundation of Shanghai(23ZR1416500);the Frontiers Science Center for Materiobiology and Dynamic Chemistry(JKVJ1231036).

语种：英文

中文关键词：Cephalosporin C;A.chrysogenum;Surfactants;Catalase;ROS

摘要：Cephalosporin C(CPC)is a critical raw material for cephalosporin antibiotics produced by Acremonium chrysogenum.During fermentation,the oxygen supply is a crucial factor limiting the efficient biosynthesis of CPC.This study demonstrated that the addition of exogenous surfactants significantly increased the dissolved oxygen(DO)level,extracellular catalase content,and final CPC titer.Consequently,we hypothesized and examined a correlation between catalase and CPC biosynthesis in A.chrysogenum through both the exogenous addition of hydrogen peroxide(H_(2)O_(2))and the endogenous modulation of the catA expression level.The results indicated that both the addition of H_(2)O_(2)and theΔcatA mutation exhibited similar fermentation trends,leading to decreased extracellular catalase activity and increased intracellular reactive oxygen species(ROS)content,which resulted in reduced CPC production.Conversely,strains that overexpress varying levels of the catA accelerated hyphal differentiation under DO-limiting conditions,reducing intracellular ROS accumulation and decreasing cellular apoptosis,which stabilized CPC yield during the later stages of fermentation.This study provides a critical foundation for further investigations into the regulatory mechanisms governing CPC biosynthesis in A.chrysogenum.