文献类型：期刊文献

英文题名：Application of Euler-Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor

作者：Liu, Yu[1];Wang, Ze-Jian[1,3];Xia, Jian-Ye[1];Haringa, Cees[3];Liu, Ya-Ping[2];Chu, Ju[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dalian Pract Biotechnol Co Ltd, Dalian, Peoples R China;[3]Delft Univ Technol, Dept Chem Engn, Julianalaan 136, NL-2628 BL Delft, Netherlands

年份：2016

卷号：114

起止页码：212

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20163002634110);WOS:【SCI-EXPANDED(收录号:WOS:000387522000024)】；

基金：This work was financially supported by the National High Technology Research and Development Program (2015AA021005), and 973 Program No. 2013CB733600. The Royal DSM and partially supported by NOW-MoST Joint Program (2013DFG32630). We also thank pharmacy corporation Practical Bio Co., Ltd. (DALIAN, China) for donating the industrial strain.

语种：英文

外文关键词：Plant cell culture; Fluid mechanics; Agitation; Power consumption; C tinctorius L. cell; Stress thresholds

摘要：The relationship between shear environment and physiological characteristics of plant cells is always a crucial and challenging aspect in the scale up of suspension cultivations. In this work, we propose a quantitative method for evaluating the lethal effects of hydrodynamic on Carthamus tinctorius L. cell with computational fluid dynamic (CFD) technology and online capacitance viable cell detection. The study towards the effect of short term shear force on C tinctorius L. cells was carried out in 5 L bioreactor under various overall shear intensities (0.02-1.82 w/kg), in which an online capacitance electrode was applied to count the dynamic vital cell. A first order kinetic model was achieved to describe the death rate in a time span of 60 min based on the capacitance. A product of the maximum shear stress and shear frequency (SSF) parameter, which is an evolution of energy dissipation/circulation function, was established to relate cell death rate to the shear environment. A good correlation between cell death kinetics and the SSF parameter was observed in the verification test in a 15 L STR bioreactor. SSF can be used to determine the maximum operating range of hydrodynamic stress, and improve the design and optimization of the of C. tinctorius L cultivation in scale-up process. (C) 2016 Elsevier B.V. All rights reserved.