文献类型：专利

英文题名：Extracting coenzyme A substance from Saccharomyces cerevisiae cells comprises inactivating the Saccharomyces cerevisiae culture to obtain treated sample and extracting the treated cell sample

作者：CHU J;HONG M;HUANG M;ZHUANG Y;CHEN C;MOU H;ZHUANG Z;LIU Z;CHEN G

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN107459545-A

申请日：2016-06-06

公开日：2017-12-12

语种：英文

收录：DERWENT

摘要：NOVELTY - Extracting coenzyme A substance from Saccharomyces cerevisiae cells comprises (i) inactivating the Saccharomyces cerevisiae culture using liquid nitrogen inactivation to obtain a treated cell sample, (ii) extracting the treated cell sample obtained in the step (i) by liquid nitrogen grinding extraction to obtain an intracellular extract containing the coenzyme A class substance. USE - The method is useful for extracting coenzyme A substance from Saccharomyces cerevisiae cells (claimed). ADVANTAGE - The method provide a basis for the metabolic analysis of the metabolic characteristics of erythromycin in the synthesis process. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for extracting organic acids from Saccharomyces cerevisiae cells, comprising: (i) inactivating the Saccharomyces carotovora culture using liquid nitrogen inactivation to obtain a treated cell sample, (ii) washing the cell sample obtained in step (i) to remove extracellular organic acids to obtain a washed cell sample AND (iii) washing the washed cell sample obtained in step (ii) with a boiling ethanol extraction method extract.