文献类型：期刊文献

英文题名：Biofabricated Nanoparticle Coating for Liver-Cell Targeting

作者：Zhang, Cheng[1,2];Qu, Xue[1,2];Li, Jinyang[1,2,3];Hong, Hua[1,2];Li, Jianbo;Ren, Jie[3];Payne, Gregory F.[4,5];Liu, Changsheng[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]Tongji Univ, Sch Mat Sci & Engn, Inst Nano & Biopolymer Mat, Shanghai 201804, Peoples R China;[4]Inst Biosyst & Biotechnol Res, College Pk, MD 20742 USA;[5]Fischell Dept Engn, College Pk, MD 20742 USA

年份：2015

卷号：4

期号：13

起止页码：1972

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000367752300008)】；

基金：The authors acknowledge the financial support from the National Basic Research Program of China (2012CB933600), the National Natural Science Foundation of China (51103043), the 111 project (B14018), Innovation Program of Shanghai Municipal Education Commission (14ZZ060), Shanghai Science and Technology Development Funds (14QA1401000), the Fundamental Research Funds for the Central Universities (WD1214056) and the United States National Science Foundation (CBET-1435957).

语种：英文

摘要：Biology routinely uses noncovalent interactions to perform complex functions that range from the molecular recognition of ligand-receptor binding to the reversible self-assembly/disassembly of hierarchical nanostructures (e.g., virus particles). Potentially, biological materials that offer such recognition and reversible self-assembly functionality can be applied to nanomedicine. Here, polysaccharides with the multifunctional polysaccharide-binding protein Concanavalin A (Con A) are coupled to create a functional nanoparticle coating. This coating is self-assembled in a layer-by-layer format by sequentially contacting a nanoparticle with Con A and the polysaccharide glycogen. In the final assembly step, a galactomannan targeting ligand is self-assembled into the coating. Evidence indicates that the mannose residues of the galactomannan backbone are responsible for assembly into the coating by Con A binding, while the galactose side chain residues are responsible for targeting to the liver-specific asialoglycoprotein receptor (ASGP-R). Binding to ASGP-R induces endocytic uptake, while the low endosomal pH triggers disassembly of the coating and release of the nanoparticle-entrapped drug. In vitro cell studies indicate that the coating confers liver-cell-specific function for both nanoparticle uptake and drug delivery. These studies extend the use of Con A to sugar-mediated and organ-specific targeting, and further illustrate the potential of biologically based fabrication for generating functional materials.