文献类型：期刊文献

英文题名：Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis

作者：Chen, Jin[1];Ni, Yi[1];Liu, Chenchen[1];Yamaguchi, Yoshinori[1,2];Chen, Qinmiao[1];Sekine, Shinichi[3];Zhu, Xifang[4];Dou, Xiaoming[1,2,4]

机构：[1]East China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Suita, Osaka 5650871, Japan;[3]Osaka Univ, Grad Sch Dent, Dept Prevent Dent, Suita, Osaka 5650871, Japan;[4]ChangZhou Inst Technol, Sch Optoelect Engn, 299 South Tongjiang Rd, Changzhou 213002, Jiangsu, Peoples R China

年份：2016

卷号：160

起止页码：425

外文期刊名：TALANTA

收录：;EI(收录号：20163202691373);WOS:【SCI-EXPANDED(收录号:WOS:000383524400054)】；

基金：The project was financially supported by the National Natural Science Foundation of China (No. 21305089), Grants-in-Aid for Scientific Research of Japan (A15H038270 [Y.Y.]), and the Fund of East China University of Science and Technology (No. YK0142119).

语种：英文

外文关键词：Short-end capillary electrophoresis; Space domain internal standard method; Oral bacteria; Polymerase chain reaction

摘要：High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R-2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/mu l, respectively. (C) 2016 Elsevier B.V. All rights reserved.