文献类型：期刊文献

英文题名：In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells

作者：Zhang, Wenchao[1,2];Wei, Xue[1,2];Wang, Qingliang[1,2];Dai, Kai[1,2,3];Wang, Jing[1,2,4];Liu, Changsheng[2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai, Peoples R China

年份：2024

期号：204

外文期刊名：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001220480600024)】；

基金：This research was supported by the Basic Science Center Program (No. T2288102) , the Key Program of the National Natural Science Foundation of China (No. 32230059) , the National Natural Science Foundation of China (No. 32301123) , the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry (No. JKVD1211002) ,the Wego Project of Chinese Academy of Sciences (No. (2020) 005) , the Project of National Facility for Translational Medicine (Shanghai) (No. TMSK-2021-134) , and the China Postdoctoral Science Foundation (No. 2022M721147) .r the Wego Project of Chinese Academy of Sciences (No. (2020) 005) , the Project of National Facility for Translational Medicine (Shanghai) (No. TMSK-2021-134) , and the China Postdoctoral Science Foundation (No. 2022M721147) .

语种：英文

摘要：Hematopoietic stem cell transplantation (HSCT) requires a sufficient number of therapeutic hematopoietic stem/progenitor cells (HSPCs). To identify an adequate source of HSPCs, we developed an in vivo osteo-organoid by implanting scaffolds loaded with recombinant human bone morphogenetic protein -2 (rhBMP-2) into an internal muscle pouch near the femur in mice. After 12 weeks of implantation, we retrieved the in vivo osteo-organoids and conducted flow cytometry analysis on HPSCs, revealing a significant presence of HSPC subsets within the in vivo osteoorganoids. We then established a sublethal model of hematopoietic/immune system injury in mice through radiation and performed hematopoietic stem cell transplantation (HSCT) by injecting the extracted osteo-organoid-derived cells into the peripheral blood of radiated mice. The effect of hematopoietic recovery was evaluated through hematological, peripheral blood chimerism, and solid organ chimerism analyses. The results confirmed that in vivo osteo-organoid-derived cells can rapidly and efficiently reconstruct damaged peripheral and solid immune organs in irradiated mice. This approach holds potential as an alternative source of HSPCs for HSCT, offering benefits to a larger number of patients.