文献类型：期刊文献

英文题名：Myricetin protects natural killer cells from arsenite induced DNA damage by attenuating oxidative stress and retaining poly(ADP-Ribose) polymerase 1 activity

作者：Ma, Huijuan[1,2];Song, Xiaodong[3];Huang, Ping[1,2];Zhang, Weiwei[1,2];Ling, Xinyue[1,2];Yang, Xiaoning[1,2];Wu, Wenwei[1,2];Xu, Huan[1,2];Wang, Wei[1,2,4,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Shanghai 200237, Peoples R China;[3]Fudan Univ, Hua Shan Hosp North, Med Lab Dept, Shanghai 201907, Peoples R China;[4]Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA;[5]Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA

年份：2021

卷号：865

外文期刊名：MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000641397400001)】；

基金：This work was funded by National Natural Science Foundation of China [grant No. 21906057 to H.X., grant No. 21738002 to W.W.] and Shanghai Science and Technology Committee through the Shanghai Sailing Program [grant No. 19YF1412500 to H.X.] .

语种：英文

外文关键词：Myricetin; NK cells; DNA damage; Oxidative stress; PARP-1; Genotoxicity

摘要：Environmental exposure to arsenite (As+3) is known to induce immunotoxicity. Natural killer (NK) cells are innate lymphoid cells act as professional killers of tumor cells. Our previous report indicated that 500 ppb As+3 drinking water exposure induced significant DNA damage in the NK cells of C57BL/6 mice. Myricetin is a plantderived flavonoid known as a strong antioxidant. In this study, daily administration of myricetin at 20 mg/kg was found to alleviate the cell population decrease and DNA damage in the NK cells of BALB/c mice exposed to 500 and 1000 ppb As+3 via drinking water. Oxidative stress and poly(ADP-ribose) polymerase 1 (PARP-1) inhibition were induced by As+3 at 1 and 2 ?M in isolated mouse NK cells in vitro, which were attenuated by 20 ?M myricetin. The mitigatory effect of myricetin on the PARP-1 inhibition in NK cells treated with As+3 was also found to be the result of its prevention of the zinc loss induced by As+3 on PARP-1. Collectively, these results demonstrated, for the first time, that myricetin could protect NK cells from As+3 induced DNA through attenuating oxidative stress and retaining PARP-1 activity, indicating that myricetin may be utilized for the prevention of the immunotoxicity induced by As+3 in NK cells.