文献类型：期刊文献

中文题名：ICR小鼠胚胎NSCs体外培养和诱导分化

英文题名：In Vitro Culture and Induced Differentiation of Neural Stem Cells from ICR Fetal Mouse

作者：邓磊[1];郭元昕[1];庄英萍[1];储炬[1];郭美锦[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2011

卷号：37

期号：5

起止页码：544

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家重点实验室开放课题(2060204);国家863计划(2007AA02Z216)

语种：中文

中文关键词：神经干细胞;无血清培养基;支持细胞;ICR小鼠;诱导分化

外文关键词：neural stem cells; serum-free medium; sertoli cells; ICR mouse; induced differentiation

摘要：利用表皮生长因子(EGF)和碱性成纤维生长因子(bFGF)的联合促进作用使原代胚胎皮层神经干细胞(Neural Stem Cells,NSCs)稳定增殖,以此来分离培养ICR小鼠胚胎NSCs,并在体外高效诱导成神经元。通过诱导分化的对照实验获得神经元分化比例最高的方法。免疫细胞化学不仅证实了培养的NSCs可表达Nestin并具有多分化潜能,还发现NSCs与支持细胞(SCs)共培养时神经元分化比例高达60%(P<5)。研究发现细胞生长因子EGF(20 ng/mL)和bFGF(20ng/mL)能有效促使NSCs在无血清条件下增殖,并且支持细胞促进其向神经元分化。
To isolate and culture the ICR fetal mouse neural stem cells（NSCs） and efficiently produce induced-neurons in vitro,this study utilised the synergistic effects of EGF and bFGF to promote the proliferation of primary fetal cortical NSCs.The approach with highest neuronal differentiation rate was confirmed through control induction experiments.Immunocytochemistry staining recorded that NSCs had the pluripotency,and the neuronal differentiation rate in pattern of co-culture NSCs and sertoli cells（SCs） was up to 60%（P〈5）.Therefore,it was proved that EGF（20 ng/mL） and bFGF（20 ng/mL） could efficiently boost the proliferation of NSCs in the serum-free medium,moreover,SCs contributed significantly to inducing NSCs into neurons.